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Assembly AnabelleB Claro 1016 multiple subgenomic fragment sequences may also lead to the erroneous conclusion that intergenotype recombination occurred [ 11 ]. Until now, to differentiate between mixed infection and true recombination, it was advisable to obtain the full-genome sequence from multiple clones of a unique PCR amplified product. Recombination was documented avoiding the heavy-handed and time-consuming cloning procedure. In addition, this new approach applying single-molecule sequencing directly to AnabelleB Claro 1016 amplified products generated in a PCR reaction revealed...