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For control and DR mice, V 1 was taken from both hemispheres, while for MD mice only V 1 contralateral to the deprived eye was used. For each sample, total RNA was extracted and processed for the microarray procedure.
MD and DR samples were compared to the control independently, each with two different computational ACTION ACTINA NEVADA W7H SERIES see Example 1: Each procedure identified single genes or gene sets that were up- or down-regulated in deprived groups versus control. This led to the identification of cellular events involved in the two models of input deprivation.
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B, C Comparison of gene expression in B dark-reared versus control and C monocularly deprived versus control animals, showing the expression levels of all probes. Gene expression is shown on a logarithmic scale.
D Heat map showing the levels of expression of representative genes that showed differential expression among those selected for our. High levels of expression correspond to brilliant red, low levels of expression correspond to dark blue see bottom of the figure for color scale.
For each group, 25 randomly chosen genes among the significant genes are shown here. Genes within each group are sorted based on their expression values.
Regulation of genes involved in excitatory and inhibitory transmission in MD and DR animals. A Heat map of the genes confirmed with semi-quantitative PCR.
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The level of expression is represented in logarithmic scale; red corresponds to maximal expression and blue to minimal expression. The genes are ranked according to their expression level after MD. The genes in the dataset are ranked according to a correlation statistic signal-to-noise ratio ; genes ACTION ACTINA NEVADA W7H SERIES after MD vs. The straight lines represent genes in the ranked list that are in the ARF pathway bottom. The running enrichment score is plotted in the upper graph top.
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Highest levels of expression correspond to brilliant red, while lowest levels of expression correspond to dark blue. The arrows highlight two pathways that are particularly enriched in DR and are discussed in the text: The insets show the running enrichment scores for these two pathways; the red ACTION ACTINA NEVADA W7H SERIES show the positions of Creb and GluRl probes ACTION ACTINA NEVADA W7H SERIES. The arrows indicate two pathways discussed in the text which are particularly enriched in MD: For each of these pathways, the insets show the running enrichment score.
Immunohistochemistry for molecules that show increased expression following DR and MD. In DR mice, the expression of three proteins: The parvalbumin gene is down-regulated in DR versus control and the immunohistochemistry shows a decrease in the number of parvalbumin-positive neurons in DR animals. Both proteins are selectively up-regulated in V 1 after 15 days of MD relative to control.
Arrows in the low magnification pictures demarcate V I. A Left: Mouse brain showing the location of V 1 black region.
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Ocular dominance index map in mouse V 1. Download the latest drivers for your Action ACTINA NEVADA W7H SERIES to keep your Computer up-to-date. Download the latest drivers for your Action ACTINA NEVADA X to keep your Computer up-to-date.